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KMID : 0379520110270020077
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한국독성학회지
2011 Volume.27 No. 2 p.77 ~ p.83
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Comparative Antitumor Activity of Different Solvent Fractions from an Auricularia auricula-judae Ethanol Extract in P388D1 and Sarcoma 180 Cells
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Reza Ahsanur
Choi Myung-Jin
Damte Dereje
Jo Woo-Sik
Lee Seung-Jin
Lee Joong-Su
Park Seung-Chun
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Abstract
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The objective of this study was to evaluate and compare the antitumor activity of different solvent fractions (ethanol, dichloromethane, ethyl acetate, butanol and water) of the Auricularia auricula-judae 70% ethanol extract on the P388D1 macrophage and sarcoma 180 cells. A dose-dependent antitumor activity of each solvent fraction (from 0.01 ㎎/㎖ to 0.3 ㎎/㎖) was shown against both cell types. These cytotoxic effects of all the tested fractions were confirmed on the MTT and SRB assays, without statistical differences each other. IC?? value of dichloromethane fraction was 94.2 ㎍/㎖ against sarcoma 180 cells lower than any other solvent fractions. The potent antitumor effect of the dichloromethane (DCM) fraction was also found against solid tumor in BALB/c mice. The splenomegaly and higher splenic index were found in tumor-bearing mice, with the DCM fraction returning to the negative control values. Thus, the results indicated the dichloromethane fraction may have potential ingredients as antitumor candidates.
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KEYWORD
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Auricularia auricula-judae, Antitumor, Solvent fractions, P388D1 macrophage cell, Sarcoma 180 cell
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